PCR Enclosures
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Sigma Aldrich Fine Chemicals Biosciences fastStartTM PCR Master
Hot start polymerase chain reaction (PCR) is effortless using the fastStart(TM) PCR Master a ready-to-use double-concentrated hot start master mix. This master mix contains fastStart Taq DNA Polymerase Roche high quality PCR-Grade nucleotides and all other reagents required for PCR and two-step reverse transcriptase (RT)-PCR on thermal cycler instruments.fastStart(TM) PCR Master accepts modified nucleotides such as Digoxigenin (DIG)-dUTP. Labeling with DIG-dUTP can be achieved by adding the modified nucleotide in a final concentration of 10 muM to the final volume.Combine this master mix with Roches Transcriptor first Strand cDNA Synthesis Kit to achieve excellent results in two-step RT-PCR.
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